The protein extracted from mature leaves was tested for mosquito larvicidal activity against third instar larvae of cx. The control were also prepared along side, this were include. Pharmacognostic evaluation and larvicidal activity of selected three ocimum species. The larrvicidal activity of mosquito and phytochemical screening of methanol extract of leaves belongs to the family meliaceae have been evaluated in the present study. Liliaceae leaf extract and bacillus sphaericus, against chikungunya vector, aedes aegypti jayapal subramaniam, a kalimuthu kovendan, a. Prevention is better than cure, so, the mosquito knockdown and larvicidal action of cl oil may be an effective step to prevent dengue virus transmission. Larvicidal bioassay the larvicidal bioactivity of the different parts of rnyzoides leaves, stem, flower, and roots extracts on the 3rd.
Cocobear, a high performance larvicidal oil, has a patentpending formulation that reduces petroleum distillates from 98. Comparisons of mosquito larvicidal activity of cinnamaldehyde congeners revealed that cinnamaldehyde exhibited the strongest mosquito larvicidal activity. The hexane, chloroform, ethyl acetate, acetone, and methanol leaf, flower and seed extracts of abrus precatorius a. The larvae were reared in dechlorinated water and fed with dog biscuits and yeast powder at the ratio of 3. The efficacy of different plant parts has been well. Lagundi against mosquito larvae a research paper presented to the curriculum and instruction services division philippine science high school central visayas campus. Mosquito larvicidal activity of hyptis suaveolens filarial. The leaves methanolic extract and the seeds petroleum ether extract of a. Mosquito is a vector of different diseases like malaria, filariasis and dengue fever.
Larviciding is a successful method of reducing mosquito population in their breeding place before they emerge as adults. Asteraceae leaf extracts against the filarial vector culex quinquefasciatus say. Larvicidal activity of anacardium occidentale against aedes aegypti. Larvicidal activity of anacardium occidentale against. Mosquito larvicidal and antimicrobial activity of synthesized nanocrystalline silver particles using leaves and green berry extract of solanum nigrum l. Larvicidal activity of methanolic extract of the leaves of. The soxhlet extraction method had the highest larvicidal effect against ae. Toddalia asiatica extracts of whole plant of citrullus colocynthis and sphaeranthus. Chemical composition and larvicidal activities of azolla.
Mosquito risk has become more acute in recent time and the death of millions of people every year due to mosquito borne diseases has resulted in the loss of socioeconomic wealth in many countries. Evaluation of mosquito larvicidal effect of carica coden. Introduction malaria is one of the most live threatening and widespread infectious diseases of our time. Also the screening of locally available medicinal plants for mosquito control would generate local employment, reduce dependence on expensive imported products and stimulate. In the present study an attempt was made to analyze the larvicidal activity of crude and ethyl acetate extracts of matured seed coat of cassia sophera against culex quinquefaciatus. Mosquito larvicidal and pupicidal activity of seaweed extracts against aedes aegypti, anopheles stephensi and culex quinquefasciatus. Evaluation of larvicidal activity of medicinal plant.
Palanisamy mahesh kumar, a kadarkarai murugan, a and william walton b. It has been estimated that about 40% of the worlds population live in. Larvicidal effect on 3rd and 4th instar larvae of aedes aegypti and. In brief, 20 number of fourth instars mosquito larvae were placed in a conical flask with 249 ml phosphate buffered saline, maintain at room. Most recent pestalotiopsis studies have been based on endophytic isolates15,23,24.
The larvicidal activity of pine oil was determined against three major urban mosquito vectors an. Below are the links to the authors original submitted files for images. Larvicidal activity of essential oils from brazilian plants. Bioactive compounds, lemongrass, culex mosquitoes, larvicidal activity, mosquito borne diseases. Mosquito control programmes largely target the larval stage at their breeding sites with larvicides 4. Extracts of a selection of plant taxa sourced in south africa were tested for larvicidal properties in an applicable assay. Laboratory colonies of mosquito larvaepupae were used for the larvicidal pupicidal activity. Welcome to niscair online periodicals repository you can now access full text articles from research journals published by csirniscair. Based on the results the authors have proposed that crude extracts of a. A comparative study of the larvicidal activity of lemongrass. Mosquito larvicidal activity of medicinal plants 231 table 5. The results indicated that the aqueous leaf extract of citrus grandis is effective against culex quinquefasciatus mosquito larvae and need to be explored for its possible use in the control of mosquito population. Sep 10, 2012 this study was initiated to establish whether any south african ethnomedicinal plants indigenous or exotic, that have been reported to be used traditionally to repel or kill mosquitoes, exhibit effective mosquito larvicidal properties. To evaluate the mosquito larvicidal activity of plant extracts.
Flyash based mosquito larvicidal formulations of bacillus thuringiensis var. Citrullus colocynthis extracts insecticidal activity pdf. Larvicidal and pupcidal efficacy of momordica charantia. The larvicidal and pupicidal bioassay were assessed by using standard who protocols 1. Methylenedioxy compounds are considered to be safe synergists that enhance the activity of active ingredients to control mosquito populations. Larvicidal activity of the formulation was also evaluated in field against anopheles, culex, and aedes mosquitoes. The larvicidal activity of the fruit extract against cx. Larvicidal and knockdown activity of citrus limetta. Larvicidal synergistic activity of plant extracts for mosquito control article pdf available in the indian journal of medical research 821. Jul 22, 2012 mosquito larvicidal activity of aloe vera family. The larvicidal activities of the three plants differ according to the plant species and part used which is supported by the presence of several bioactive chemicals. Such plant extracts have been investigated for their ability to kill a number of mosquito species known to be malaria vectors 14,1719.
Cinnamaldehyde had the best mosquito larvicidal activity, with an lc 50 of 29 ppm lc 90 48 ppm against a. The activity of phospholipase decreases with increase in size of the seeds. Evaluation of larvicidal potential of flavonoid extracted. Evaluation of mosquito larvicidal effect of carica papaya against aedes aegypti p. Synthesis, crystal structure and larvicidal activity of. Cl oil was found to be effective against mosquitoes and larvae. Larvicidal effects of endophytic and basidiomycete fungus. They function as general toxicants, growth and reproductive inhibitors, repellents and. Larvicidal activity of some plant extracts against mosquitoes.
Larvicidal efficacy of aqueous extracts of citrus grandis. Jun 10, 2004 cinnamaldehyde had the best mosquito larvicidal activity, with an lc 50 of 29 ppm lc 90 48 ppm against a. Societys college of pharmacy, ii block, rajajinagar, bangalore560 010, karnataka, india. Larvicidal activity of vernonia cinerea journal of biopesticides, 4 1. Seven naturally occurring compounds from piper nigrum and p. Larvicidal activity of medicinal plant extracts against. Chrysanthemum indicum is a traditional herb used to treat various disorders, hypertension symptoms and several infectious diseases in korean and chinese medicine 12. Introduction mosquitoes as holometabolous insects have a complex life cycle interspersed between the aquatic immature and. Larvicidal activity of neem oil azadirachta indica.
Further investigations are needed to explore the larvicidal activity of acetone and petroleum ether aerial extract of this plant against a wide range of mosquito species and also the active ingredients of the extract responsible for larvicidal activity should be identified. Mosquito larvicidal potential of medicinal plants springerlink. Mosquito larvicidal efficay of acorus calamus extracts. For detailed characterization of mosquito larvicidal activity, the lc 50 and lc 95, 24 h posttreatment of the native larvicidal bt isolates and btih14 reference strain were established using bioassays conducted on thirdinstar larvae of an. It has been increasing development of resistance to synthetic chemicals, so plant extracts may be the alternate source of mosquito control agent. Mosquito larvicidal activity of aqueous extracts of long pepper piper retrofractum vahl from thailand uruyakorn chansang 1, nayer s. Most of them exhibited higher mosquito larvicidal activity. The screening of local medicinal plants for mosquito larvicidal activity may. Screening of selected ethnomedicinal plants from south africa. Larvicidal, antipyretic and antiplasmodial activity of. Mosquito larvicidal activities of solanum villosum berry extract against the dengue vector stegomyia aegypti. Mosquito larvicidal and antimicrobial activity of protein. Globally, there is a prompt awareness going on and always desired to use natural, ecofriendly compounds for larvicidal activity.
Mosquito larvicidal activity the methanol and ethanol flower extract of lantana camara was found to have higher rate of larvicidal rate against aedes aegypti, where as in the culex quinquefasciatus variety, the concentration of extracts have to be increased for better larvicidal effect m. Plasmodium spp, the vector mosquito has to be controlled. Larvicidal activity of few select indigenous plants of north. Tinospora rumphii leaf extract is the most effective mosquito larvicide which is manifested by the highest percentage mortality on the larvae of 90% and 93%. Larvicidal, pupicidal, repellent and adulticidal activity of citrus sinensis orange peel extract against anopheles stephensi, aedesaegypti and culex quinquefasciatus diptera. Larvicidal activity of endophytic fungal extract of. Eggs are laid on the surface of water culex and anopheles types or damp soil that is soon to flood aedes type. Govindarajan m, sivakumar r, rajeswary m, veerakumar k 20b mosquito larvicidal activity of thymol from essential oil of coleus aromaticus benth. This has necessitated the need for a research and development of ecofriendly. Pdf larvicidal property of green synthesized silver. Plant extracts as potential mosquito larvicides pdf. This study suggests that the diamide derivatives exhibited good effective against mosquito. A practical synthetic route to amide derivatives by the reaction of amide with another acid is presented. Evaluation of mosquito larvicidal activities of seed coat.
Characteristics and classification of larvicidal fishes. The plant extract showed larvicidal and pupicidal ef fects after 24 h of exposure. Tinospora rumphii leaf extract is the most effective mosquito larvicide which is manifested by the highest percentage mortality on the larvae of 90% and 93% after 24 and 48 hours respectively. All relevant data are within the paper and its supporting information files. Results the larvicidal activity of azolla pinnata plant extracts evidently affected the early 4th instar larvae of aedes aegypti mosquito vectors.
Larvicidal and mosquito repellent activities of pine pinus. Especially compound 6r displayed relatively good activity to reach 70% at 2. Mulla2 1division of medical entomology, national institute of health, department of medical. Larvicidal assay in the larvidal assay, third and fourth instar larvae of aedes aegypti and culex quinquefciatus were exposed to test concentrations of 50, 100,150,200 mg of methanol and ethanol extracts of leaf of azadirachta indica in 100ml of water. Larvicidal activity of few select indigenous plants of.
Vasugi abstract the development of resistance to chemical insecticides among mosquito species has been considered as a setback in vector control. Mosquito larvicidal activities of naturally occurring. Laboratory colonies of mosquito larvaepupae were used for the larvicidalpupicidal activity. Mosquito is a vector for serious human diseases like dengue fever, hemaorrhagic dengue fever and chikungunya. Larvicidal activity of endophytic fungal extract of cochliobolus spicifer pleosporales. The larvicidal activity was done as per guideline of world health organization 14 with modifications. Mosquito larvicidal and antimicrobial activity of synthesized nanocrystalline silver particles using leaves and green berry extract of solanum. Larvicidal activity of medicinal plant extracts against aedes. The aims of the present study were to examine products of the amazon biodiversity and to identify fungi with potential larvicidal activity to control mosquitovectored diseases. Highly effective, field trials confirm 100% control inside of 2 hours, cocobear is appropriate for a wide variety of aquatic habitats. Pdf larvicidal, pupicidal, repellent and adulticidal. The high larvicidal activity of tinospora rumphii leaf is supported by the abundance of phytochemicals which show synergestic effects in terms of larvicidal action to mosquito larvae.
The current revise has opened up prospects for large scale extraction of active ingredients of plant origin for effective mosquito control. The larvicidal and mosquitocidal activities of ethanolic water mixture extract of a. Later 1 ml of the dilution was made up to 250 ml with distilled water to. Madhura mukadam department of zoology, gogate jogalekar college, ratnagiri, maharastra, india abstractmosquitoes are the major vector for the transmission of malaria, dengue fever, yellow fever, filariasis, schistosomiasis and japanese encephalitis. Pdf larvicidal activity of medicinal plant extracts.
Zahiri 2, jaree bansiddhi 3, thidarat boonruad 3, pratom thongsrirak 3, jiranuch mingmuang3, nipa benjapong1, and mir s. Rivers, streams, lakes, reservoirs, tanks, ponds, irrigation canals, multipurpose dams, paddy fields and ditches provides an immense scope, perhaps richest in the world for the propagation and production of edible fishes. This technology uses bacillus thuringiensis as biocontrol agents for mosquitoes the wdp 40% at 2. Mosquito larvicidal activity of aqueous extracts of long. Pharmacognostic evaluation and larvicidal activity of. The 15 % cl oil exhibited highest percentage of mortality equal to 82. At the same time it is necessary to control bacteria by synthetic or natural means plant products.
It was seen that pe and mc extracts exhibited higher larvicidal activity compared to ma and water table 1. Mosquitoes are flying, biting insects that develop in water during their immature stages. Evaluation of mosquito larvicidal activities of seed coat extract of cassia sophera l. Hence the present study was designed to screen the effect of mosquito larvicidal and antimicrobial activitiy of protein isolated from matured leaves of solanum villosum against mosquito immatures and some pathogenic bacteria. Keywords cochliobolus spicifer larvicidal activity mosquitoes zebrafish introduction plants may serve as a reservoir of large numbers of organisms such as bacteria, insects, nematodes, protozoa, or fungi that can live endophytically within plant tissues mul ler and doring 2009.
Pdf larvicidal synergistic activity of plant extracts for. The present study investigated the larvicidal and pupicidal activity against the first to fourth instar lavae and pupae of the laboratoryreared mosquitoes, an. Green synthesis of silver nanoparticles using solanum. A large number of plant products have been reported to have mosquito larvicidal andor repellent activity against adult mosquitoes. Larvicidal activity of selected plant extracts against the. Methanolic seed extract on the other hand, exhibited a higher degree of potency compared with the aqueous seed. Asteraceae leaf extracts against the filarial vector culex quinquefasciatus say diptera. The chemicals derived from plants have been projected as weapons for future mosquito control programs.
They function as general toxicants, growth and reproductive inhibitors, repellents and deterrents, sukumar et al. Larvicidal activity of selected plant extracts against. Full text facility is provided for all nineteen research journals viz. Review study on larvicidal and mosquito repellent activity. Flyash based mosquito larvicidal formulations of bacillus. Mosquito larvicidal activity detection bioassays initial toxicity of various extracts was assessed at a single dose of 500 gml against the test mosquito species. Review study on larvicidal and mosquito repellent activity of. Mosquito repellent activities of ocimum volatile oils. Mosquito larvicidal formulation from bacillus thuringiensis var. It was seen that pe and mc extracts exhibited higher larvicidal activity compared to ma and water. Larvicidal activity, resistance to insecticides, control. Synthesis, crystal structure and larvicidal activity of novel.
Extracts of annona species are potential anti mosquito. Antiplasmodial, larvicidal, antipyretic activity, medicinal plants. B ased on the results the authors have proposed that crude extracts of a. Mosquitos transmit human diseases including malaria, dengue fever, yellow fever, and encephalitis. Chemical composition and mosquito larvicidal activity of.